Mutant influenza A virus nucleoprotein is preferentially localized in the cytoplasm and its immunization in mice shows higher immunogenicity and cross-reactivity
作者： Kenji Ohba , Shinsuke Yoshida , Md. Zahidunnabi Dewan , Hiromi Shimura , Nozomi Sakamaki
DOI: 10.1016/J.VACCINE.2007.02.074
关键词: Orthomyxoviridae 、 Virus 、 Vaccination 、 Antigenic drift 、 Hemagglutinin (influenza) 、 Virology 、 Influenza A virus 、 Biology 、 DNA vaccination 、 Immunogenicity 、 Public Health, Environmental and Occupational Health 、 General Immunology and Microbiology 、 Molecular medicine 、 General Veterinary 、 Infectious Diseases
摘要: Many influenza vaccines targeted to hemagglutinin (HA) show efficient immunogenicity for protecting subjects against virus infection. Major antigenic changes HA molecules can help develop resistance HA-targeted vaccines. DNA encoding conserved antigens protect animals diverse subtypes, but their potency requires further improvement. We generated a DNA-based nucleoprotein (NP)-targeted vaccine using an N-terminal mutant of NP (NPm) that efficiently localized in the cytoplasm, and examined immune responses mice immunized with NPm or wild-type (WT) vaccine. Importantly, showed 1.5-2-fold higher than WT mice. Furthermore, vaccination protected lethal challenge viruses cross-reactivity toward heterologous viruses. Therefore, may contribute development protective immunity through its ability stimulate cellular immunity.
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