Efficient and Qualitatively Distinct MHC Class I-Restricted Presentation of Antigen Targeted to the Endoplasmic Reticulum
作者： Tatiana N. Golovina , E. John Wherry , Timothy N. J. Bullock , Laurence C. Eisenlohr
DOI: 10.4049/JIMMUNOL.168.6.2667
关键词: Antigen 、 MHC class I 、 Immunology 、 Glycoprotein 、 Epitope 、 Cell biology 、 Endoplasmic reticulum 、 Antigen processing 、 Biology 、 Proteasome inhibitor 、 Antigen presentation
摘要: For most nascent glycoprotein Ags, the MHC class I-restricted processing pathway begins in endoplasmic reticulum (ER). From this location, they are translocated to cytosol for degradation by proteasome. A reasonable assumption is that of exocytic Ags less efficient than cytosolic due requirement additional handling, but pathways two types proteins otherwise similar. To test this, we compared presentation three epitopes within influenza nucleoprotein (NP) when Ag targeted or ER. Surprisingly, under conditions limited expression, proteasome-dependent comparable NP ER while a third negatively impacted. Furthermore, epitope unaffected addition proteasome inhibitor expressed significantly enhanced expressed. These results indicate delivery need not preclude and ER-targeted qualitatively distinct.
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