Leucine Aminopeptidase Is Not Essential for Trimming Peptides in the Cytosol or Generating Epitopes for MHC Class I Antigen Presentation

摘要: To detect viral infections and tumors, CD8+ T lymphocytes monitor cells for the presence of antigenic peptides bound to MHC class I molecules. The majority I-presented are generated from cleavage cellular proteins by ubiquitin-proteasome pathway. Many oligopeptides produced this process too long stably bind molecules require further trimming presentation. Leucine aminopeptidase (LAP) is an IFN-inducible cytosolic that can trim precursor mature epitopes has been thought play important role in Ag examine LAP generating vivo, we LAP-deficient mice cell lines. These mutant viable grow normally. not reduced under basal conditions or after stimulation with IFN. Similarly, there no reduction presentation full-length constructs overall supply even After infection, generate normal CTL responses seven three different viruses. data demonstrate essential enzyme most reveal redundancy function aminopeptidases.