Manganese mutagenesis in yeast. II. Conditions of induction and characteristics of mitochondrial respiratory deficient Saccharomyces cerevisiae mutants induced with manganese and cobalt.

摘要: Manganese and cobalt are capable of inducing ρ − mutations* in non-growing cells Saccharomyces cerevisiae , but their mutagenic action is much stronger growing cells. At a given concentration manganese can be either strongly or non-mutagenic, depending on the cell density. Most mutants induced with considerable proportion those suppressive and/or transmit drug resistance markers, so they must still carry mitochondrial DNA. Cobalt decrease suppressiveness low efficiency eliminate markers from established clones.