Amperometric detection of DNA hybridization on a gold surface depends on the orientation of oligonucleotide chains.
作者： Tibor Hianik , Victor Gajdos , Roland Krivanek , Tatjana Oretskaya , Valeri Metelev
DOI: 10.1016/S0302-4598(00)00130-6
关键词: Chemisorption 、 Oligonucleotide 、 DNA 、 Amperometry 、 Conductance 、 Analytical chemistry 、 Chemistry 、 Quartz crystal microbalance 、 Crystallography 、 DNA–DNA hybridization 、 Thiol
摘要: Abstract We tested the possibility of amperometric detection DNA hybridization on a gold surface influenced by immobilization oligonucleotide giving different orientations single stranded relative to surface. The sensor was fabricated chemisorption 18-mer modified phosphorothioate group either at its 3′ or both and 5′ terminal. After support, immersed in 11-mercaptoundecanoic acid (MUA) solution. Further MUA resulted approximately 10-fold increase resistance organic layer. Addition complementary an conductivity for oriented perpendicular support (DNA with one thiol group), while conductance decreased parallel (with groups terminals). non-complementary chain slight decrease no change conductivity. process types is not cooperative can be described Langmuir isotherms. event has been confirmed mass using quartz crystal microbalance technique.
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