Microbiota formed on attached stainless steel coupons correlates with the natural biofilm of the sink surface in domestic kitchens.

摘要: Stainless steel coupons are frequently used in biofilm studies the laboratory, as this material is commonly food industry. The attached to different surfaces create a "natural" be studied further laboratory trials. However, little has been done investigate how well microbiota on such represents surrounding environment. sink wall and new stainless for 3 months 8 domestic kitchen sinks was investigated by next-generation sequencing (MiSeq) of 16S rRNA gene derived from DNA RNA (cDNA), plating identification colonies. mean number colony-forming units about 10-fold higher than surfaces, more variation bacterial counts between kitchens seen coupons. majority biofilms dominated Moraxellaceae (genus Moraxella/Enhydrobacter) Micrococcaceae Kocuria). results demonstrated that mainly due differences (38.2%), followed nucleic acid template (DNA vs RNA) (10.8%), only 5.1% result surfaces. smaller samples based their DNA. Overall, our suggest suited model dominating part natural environment and, furthermore, suitable downstream studies.