Isoelectric focusing of human hemoglobin: its application to screening, to the characterization of 70 variants, and to the study of modified fractions of normal hemoglobins
作者： P Basset , Y Beuzard , MC Garel , J Rosa
DOI: 10.1182/BLOOD.V51.5.971.971
关键词: Chemistry 、 Isoelectric focusing 、 Molecular biology 、 Screening method 、 Polyacrylamide gel electrophoresis 、 Abnormal hemoglobin 、 Chromatography 、 Mutant 、 Whole blood 、 Hemoglobin 、 Resolution (mass spectrometry)
摘要: Isoelectric focusing on slabs of acrylamide gel was adapted for the screening abnormal hemoglobins, characterization 70 human variants, and study minor fractions normal hemoglobin. The method as fast inexpensive conventional techniques, allowed simultaneous analysis some 50 samples whole blood, yielded resolution superior to that obtained by other methods with hemolysates. Among 31 mutants could not be separated from HbS cellulose acetate electrophoresis. technique electrofocusing us distinguish between most variants. Only one mutant, Hb Galveston, confused HbS. Koln, frequent unstable exhibited a special pattern. HbA1C HbA. Preliminary results indicate quantitation scanning is feasible.
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