Effects of Methanol, Atrazine, and Copper on the Ultrastructure of Pseudokirchneriella Subcapitata (Selenastrum Capricornutum).

作者: David C. Garrett

DOI:

关键词: NucleusOrganelleBiochemistrySelenastrumChloroplastVolume (thermodynamics)PhosphateBiologyAtrazineToxicity

摘要: The toxicity of methanol, atrazine, and copper to Pseudokirchneriella subcapitata (Korshikov) Hindak historically referred as Selenastrum capricornutum Printz were determined following 96 hrs growth in a modified Goram's media. Methanol atrazine inhibited fluorescence readings the cultures by 50% (IC50) at concentrations 2% 82 µg/l respectively. These values compared favorably other published reports. IC50 for was 160 which is substantially higher than reported values. This understandable because high chelating capacity use stereologically derived relative volume chloroplasts, mitochondria, lipid bodies, phosphate nucleus investigated determine if it could be used sensitive endpoint tests. fractions chloroplasts mitochondria normally distributed control cells while nuclei, bodies not. most dominate organelle occupying mean 46% occupied 3%. median 7% rare section profile no meaningful calculated. Up 82nd percentile sectioned profiles contained recognizable bodies. not No significant differences detected or any treatment. Limited only that appear biologically occurred methanol treated where an increase bodies' apparently concentration dependent. Significant do significant.

参考文章(72)
AG Payne, RH Hall, A Method for Measuring Algal Toxicity and Its Application to the Safety Assessment of New Chemicals Proceedings and Report of Meeting on Agrochemical Residue-Biota Interactions FAO/IAEA, 1978.. pp. 171- 180 ,(1979) , 10.1520/STP34886S
Sue Ellen Frederick, Eldon H. Newcomb, CYTOCHEMICAL LOCALIZATION OF CATALASE IN LEAF MICROBODIES (PEROXISOMES) Journal of Cell Biology. ,vol. 43, pp. 343- 353 ,(1969) , 10.1083/JCB.43.2.343
E. STEEMANN NIELSEN, S. WIUM-ANDERSEN, The Influence of Cu on Photosynthesis and Growth in Diatoms Physiologia Plantarum. ,vol. 24, pp. 480- 484 ,(1971) , 10.1111/J.1399-3054.1971.TB03522.X
Salwa A. Shehata, Osama A. Aly, Hoda Farag, Effect of Bladex, Etazine, Dicryl, and Baylucide on the growth of Scenedesmus Environment International. ,vol. 10, pp. 225- 234 ,(1984) , 10.1016/0160-4120(84)90239-3
A. El Jay, Effects of organic solvents and solvent-atrazine interactions on two algae, Chlorella vulgaris and Selenastrum capricornutum. Archives of Environmental Contamination and Toxicology. ,vol. 31, pp. 84- 90 ,(1996) , 10.1007/BF00203911
J. C. Fernandes, F. S. Henriques, Biochemical, physiological, and structural effects of excess copper in plants The Botanical Review. ,vol. 57, pp. 246- 273 ,(1991) , 10.1007/BF02858564
R. Frank, G.J. Sirons, Atrazine: Its use in corn production and its loss to stream waters in Southern Ontario, 1975–1977 Science of The Total Environment. ,vol. 12, pp. 223- 239 ,(1979) , 10.1016/0048-9697(79)90088-3
G. F. Daniel, A. H. L. Chamberlain, Copper Immobilization in Fouling Diatoms Botanica Marina. ,vol. 24, pp. 229- 244 ,(1981) , 10.1515/BOTM.1981.24.4.229
Jerome R. Cain, R. Lawrence Klotz, Francis R. Trainor, Raymond Costello, Algal assay and chemical analysis: A comparative study of water quality assessment techniques in a polluted river Environmental Pollution. ,vol. 19, pp. 215- 224 ,(1979) , 10.1016/0013-9327(79)90043-0