Complementary Imaging of Silver Nanoparticle Interactions with Green Algae: Dark-Field Microscopy, Electron Microscopy, and Nanoscale Secondary Ion Mass Spectrometry

摘要: Increasing consumer use of engineered nanomaterials has led to significantly increased efforts understand their potential impact on the environment and living organisms. Currently, no individual technique can provide all necessary information such as size, distribution, chemistry in complex biological systems. Consequently, there is a need develop complementary instrumental imaging approaches that enhanced understanding these “bio-nano” interactions overcome limitations techniques. Here we used multimodal approach incorporating dark-field light microscopy, high-resolution electron nanoscale secondary ion mass spectrometry (NanoSIMS). The aim was gain insight into bio-nano surface-functionalized silver nanoparticles (Ag-NPs) with green algae Raphidocelis subcapitata, by combining fidelity, spatial resolution, elemental identification offered three techniques, respectively. Each ...