In vitro antiprotozoal activity and cytotoxicity of extracts and fractions from the leaves, root bark and stem bark of Isolona hexaloba.

摘要: Ethnopharmacological relevance Isolona hexaloba (Pierre) Engl. and Diels (Annonaceae) is traditionally used in D.R. Congo against parasitic diseases including malaria. Materials methods Two crude aqueous extracts, 3 methanol extracts 80% ethanol from the leaves, root bark stem together with 12 subfractions were evaluated vitro for their antiprotozoal activity Trypanosoma brucei brucei, T. cruzi, Leishmania infantum chloroquine pyrimethamine resistant K1 strain of Plasmodium falciparum. Their cytotoxic effects MRC-5 cell lines also assessed. Results Results indicated that most pronounced activities b. recorded (IC50=1.97 µg/ml; SI>32.49) leaves (IC50=2.65 SI>24.15). Three samples displayed good cruzi: extract (IC50=8.33 SI>3.92), its petroleum ether fraction (IC50=8.50 SI=2.52) (IC50=9.31 SI=3.46). The exhibited a selective L. (IC50=2.00 SI>32). (IC50=6.35 SI>10.10) 2 dichloromethane soluble fractions (IC50=6.96 SI=6.1) (IC50=8 SI>8.00) showed selectivity infantum. active falciparum (0.92 µg/ml) fractions: alkaline (IC50=0.27 µg/ml), 90% (0.90 (1.04 respectively, promising indexes 35