Dihydromyricetin reduced Bcl-2 expression via p53 in human hepatoma HepG2 cells.
作者： Shixing Wu , Bin Liu , Qingyu Zhang , Jie Liu , Wei Zhou
DOI: 10.1371/JOURNAL.PONE.0076886
关键词: Signal transduction 、 Cancer cell 、 Gene expression 、 MTT assay 、 Bcl-2-associated X protein 、 Apoptosis 、 Molecular biology 、 Cell growth 、 Small interfering RNA 、 Biology
摘要: Dihydromyricetin (DHM) is a major active ingredient of flavonoids compounds. It exhibited anticancer activity and induced apoptosis in human hepatocellular carcinoma HepG2 cells according to our previous data. In this study, we investigated whether p53 involved DHM-triggered viability inhibition induction cancer cells. MTT [3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] assay was employed evaluate the after DHM treatment. Meanwhile, small interfering RNA (siRNA) adopted silence expression. Protein level Bax/Bcl-2 were evaluated by western blot analysis. Cell counting showed that inhibited cell growth effectively time- dose-dependent manner. P53 expression significantly increased treatment, whereas Bcl-2 reduced potently. Furthermore, co-treatment with Pifithrin-α (PFT-α, inhibitor), reversed. The Bax no significant change, which also observed silence. These findings defined supported novel function could induce up-regulating via signal pathway.
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