The mechanism of sex-specific splicing at the doublesex gene is different between Drosophila melanogaster and Bombyx mori.

摘要: We have previously reported that Bmdsx, a homologue of the sex-determining gene, doublesex (dsx), was found to be sex-specifically expressed in various tissues at larval, pupal, and adult stages silkworm, Bombyx mori, alternatively spliced yield male- female-specific mRNAs. To reveal sex-specific differences splicing patterns Bmdsx pre-mRNA, genomic sequence determined compared with cDNA sequences. The open reading frame (ORF) consisted five exons. Exons 3 4 were specifically incorporated into female type mRNA. On other hand, exon 2 5 produce male mRNA Bmdsx. As case Drosophila dsx, OD2 domain separated by intron sex-independent sex-dependent regions. Sex-specific occurred equivalent positions dsx gene. However, unlike introns showed no weak 3' splice sites, TRA/TRA-2 binding site related sequences not exon, nor even any regions Moreover, an vitro reaction consisting HeLa cell nuclear extracts female-type represented default splicing. These findings suggest structural features pre-mRNA are similar those but regulation alternative is different.