High-resolution large-scale mosaic imaging using multiphoton microscopy to characterize transgenic mouse models of human neurological disorders.

摘要: The thorough characterization of transgenic mouse models human central nervous system diseases is a necessary step in realizing the full benefit using animal to investigate disease processes and potential therapeutics. Because labor- resource-intensive nature high-resolution imaging, detailed investigation possible structural or biochemical alterations brain sections has typically focused on specific regions interest as determined by researcher priori. For example, Parkinson's researchers often focus imaging expected exhibit pathology such substantia nigra striatum. limitations acquiring storing data, additional data contained specimen not usually acquired disseminated/reported research community. Here we present method large at close resolution limit light microscopy mosaic technique conjunction with multiphoton microscopy. These maps are being used characterize several genetically modified neurological filling information “gap” among techniques magnetic resonance electron microscopic analysis.