Ionic Self‐Assembled Platform of Perylenediimide–Sodium Dodecylsulfate for Detection of Spermine in Clinical Samples

摘要: The detection and quantification of spermine in clinical practice is important for early diagnosis many diseases. Chromatographic immunoassay-based methods are commonly used. However, a fluorescence-based assay could provide real-time detection. Herein, the synthesis aggregation properties dicationic perylene probe (N1 -dodecyl-N3 -(4-phenyl)benzimidazolium-functionalized perylenediimide (DAB-PDI)) used to develop fluorescent "turn-on" ensemble discussed. fluorescence DAB-PDI (10 μm, Φ=0.55) efficiently quenched by negatively charged sodium dodecylsulfate (SDS) through formation ionic self-assembled aggregates (charge ratio negative (N) SDS positive (P) (N/P)=9). This self-assembly between has been characterized using photophysical, microscopic, dynamic light scattering, isothermal titration calorimetry, HRMS techniques. addition this solution results breakdown DAB-PDI-SDS owing strong binding with and, as result, recovered. exhibits high sensitivity selectivity water, urine, blood serum. lowest limit 27.5 nm, which at least about 36 times lower than that required cancer (1 10 μm urinary spermine).