Evaluation of the double antibody-solid phase radioimmunoassay technique in plasma LH and FSH and urinary LH measurements.
作者： S. L. Karonen , P. Lähteenmäki , U. Hohenthal , H. Adlercreutz
DOI: 10.1080/00365517809156076
关键词: Urinary system 、 Peptide hormone 、 Lactoperoxidase 、 Solid phase radioimmunoassay 、 Chemistry 、 Urine 、 Internal medicine 、 Endocrinology 、 Radioimmunoassay 、 Reference values 、 Double antibody
摘要: Double antibody-solid phase (DASP) radioimmunoassay methods for plasma LH and FSH urinary were developed carefully evaluated as to their reliability practicability. The peptide hormones iodinated enzymatically with immobilized lactoperoxidase which resulted in pure stable products of unchanged immunoreactivity. sensitivities these assay are 0.02, 0.17 0.20 mIU/tube (MRC 68/40) 68/39) (IRP-HMG, urinary), respectively. Interassay coefficients variation obtained over a 6–18 month period 14.2, 14.7 12.8%, latter values assays from one level pool samples, the value is mean those two pools different levels. Plasma reference using about 1.8–2.9 times higher than cited other types radioimmunoassay. However, urine similar thos...
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