Microbial metabolic exchange in 3D

摘要: Mono- and multispecies microbial populations alter the chemistry of their surrounding environments during colony development thereby influencing multicellular behavior interspecies interactions neighboring microbes. Here we present a methodology that enables creation three-dimensional (3D) models chemotype can be correlated to phenotype through multimodal imaging analysis. These are generated by performing matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (IMS) on serial cross-sections colonies grown 8 mm deep agar, registering data sets each section in MATLAB create model, then superimposing model with photograph themselves. As proof-of-principle, 3D were used visualize metabolic exchange between Bacillus subtilis Streptomyces coelicolor, as well as, Candida albicans Pseudomonas aeruginosa. The resulting able capture depth profile secreted metabolites within agar medium revealed properties certain signals previously not observable using two-dimensional MALDI-TOF IMS. Most significantly, capable mapping unobserved chemical distributions array sub-surface hyphae C. how this is altered presence P. aeruginosa, an opportunistic pathogen known virulence albicans. It was determined triggered increased rhamnolipid production which turn inhibiting embedded hyphal growth produced beneath at ambient temperature.