作者: Michael Williams , Edwin A. Risley
DOI: 10.1111/J.1471-4159.1979.TB04553.X
关键词: gamma-Aminobutyric acid 、 Ligand (biochemistry) 、 Binding site 、 Muscimol 、 Kinetics 、 Agonist 、 Cerebellum 、 GABA receptor 、 Chemistry 、 Biochemistry
摘要: — The binding of [3H]muscimol, a potent GABA agonist, to crude synaptic membranes prepared from rat brain was studied using filtration method isolate membrane-bound ligand. Specific found be saturable and occurred two sites Kd5 5 30 nm. Binding Na+-independent enhanced by both freezing Triton treatment. Regional subcellular distribution studies pharmacological characterization specific [3H]muscimol are consistent with the receptor.