Site-Directed Hydrophobic Labeling of Membrane Proteins: NBD-Modification of Bacteriorhodopsin
作者： H. Sigrist , E. Kislig , P. Allegrini
DOI: 10.1007/978-3-642-70010-1_10
关键词: Biological membrane 、 Cyanogen bromide 、 Membrane 、 Bacteriorhodopsin 、 Membrane protein 、 Peripheral membrane protein 、 Transmembrane protein 、 Biophysics 、 Chemistry 、 Lipid bilayer
摘要: Membrane proteins which are involved in the signal transmittance through a biological membrane or participate transfer of solutes across cell membranes presumed to span lipid bilayer. The proteins’ transmembrane disposition can be assayed by various techniques including labeling procedures with hydrophilic chemical reagents, enzyme-mediated modifications, protease accessibility and antibody interaction. Convincing evidence for intramembraneous protein arises from hydrophobic studies. Those segments intrinsic that close association hydrocarbon core bilayer may identified using photogenerated reagents (Gitler Bercovici 1980, Brunner Semenza 1981, Ross et al. 1982).
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sci-hub.st 参考文章(15)
Dieter Oesterhelt, Walther Stoeckenius, Isolation of the cell membrane of Halobacterium halobium and its fractionation into red and purple membrane Methods in Enzymology. ,vol. 31, pp. 667- 678 ,(1974) , 10.1016/0076-6879(74)31072-5
C.Y. Lai, [26] Detection of peptides by fluorescence methods Methods in Enzymology. ,vol. 47, pp. 236- 243 ,(1977) , 10.1016/0076-6879(77)47028-9
A H Ross, R Radhakrishnan, R J Robson, H G Khorana, The transmembrane domain of glycophorin A as studied by cross-linking using photoactivatable phospholipids. Journal of Biological Chemistry. ,vol. 257, pp. 4152- 4161 ,(1982) , 10.1016/S0021-9258(18)34699-4
Stuart J. FERGUSON, William J. LLOYD, Michael H. LYONS, George K. RADDA, The mitochondrial ATPase. Evidence for a single essential tyrosine residue. FEBS Journal. ,vol. 54, pp. 117- 126 ,(1975) , 10.1111/J.1432-1033.1975.TB04120.X
P B Ghosh, M W Whitehouse, 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole: a new fluorigenic reagent for amino acids and other amines Biochemical Journal. ,vol. 108, pp. 155- 156 ,(1968) , 10.1042/BJ1080155
Peter Roland ALLEGRINI, Hans SIGRIST, Johann SCHALLER, Peter ZAHLER, Site‐Directed Fluorogenic Modification of Bacteriophodopsin by 7‐Chloro‐4‐nitrobenz‐2‐oxa‐1,3‐diazole FEBS Journal. ,vol. 132, pp. 603- 608 ,(1983) , 10.1111/J.1432-1033.1983.TB07406.X
D.J. Birkett, N.C. Price, G.K. Radda, A.G. Salmon, The reactivity of SH groups with a fluorogenic reagent FEBS Letters. ,vol. 6, pp. 346- 348 ,(1970) , 10.1016/0014-5793(70)80095-3
H. R. Kaback, Jen-Shiang Hong, Giovanna Ames, Membranes and Transport CRC Critical Reviews in Microbiology. ,vol. 2, pp. 333- 376 ,(1973) , 10.3109/10408417309108389
Richard Henderson, Janet S. Jubb, Susan Whytock, Specific labelling of the protein and lipid on the extracellular surface of purple membrane. Journal of Molecular Biology. ,vol. 123, pp. 259- 274 ,(1978) , 10.1016/0022-2836(78)90325-X
Carlos Gitler, Tuvia Bercovici, Use of lipophilic photoactivatable reagents to identify the lipid-embedded domains of membrane proteins. Annals of the New York Academy of Sciences. ,vol. 346, pp. 199- 211 ,(1980) , 10.1111/J.1749-6632.1980.TB22100.X