Malaria Parasite Invasion of the Mosquito Salivary Gland Requires Interaction between the Plasmodium TRAP and the Anopheles Saglin Proteins
作者： Anil K Ghosh , Martin Devenport , Deepa Jethwaney , Dario E Kalume , Akhilesh Pandey
DOI: 10.1371/JOURNAL.PPAT.1000265
关键词: Mimotope 、 Anopheles gambiae 、 Anopheles 、 Molecular biology 、 Salivary gland 、 Binding domain 、 Plasmodium 、 Plasmodium falciparum 、 Plasma protein binding 、 Biology 、 Immunology 、 Genetics 、 Microbiology 、 Parasitology 、 Virology
摘要: SM1 is a twelve-amino-acid peptide that binds tightly to the Anopheles salivary gland and inhibits its invasion by Plasmodium sporozoites. By use of UV-crosslinking experiments between target protein, we have identified saglin, as receptor for SM1. Furthermore, an anti-SM1 antibody, determined mimotope sporozoite Thrombospondin Related Anonymous Protein (TRAP). TRAP saglin with high specificity. Point mutations in TRAP's binding domain A abrogate binding, competed peptide. Importantly, vivo down-regulation expression results strong inhibition invasion. Together, suggest saglin/TRAP interaction crucial
elsevier.com
fiocruz.br
researchgate.net 
core.ac.uk 参考文章(54)
Van Regenmortel, Synthetic Polypeptides As Antigens ,(1988)
Thomas J Templeton, David C Kaslow, Cloning and cross-species comparison of the thrombospondin-related anonymous protein (TRAP) gene from Plasmodium knowlesi, Plasmodium vivax and Plasmodium gallinaceum1Note: Nucleotide sequence data reported in this paper have been submitted to GenBank™ with the accession numbers PgTRAP: U64899; PkTRAP: U64900; and PvTRAP: U64901.1 Molecular and Biochemical Parasitology. ,vol. 84, pp. 13- 24 ,(1997) , 10.1016/S0166-6851(96)02775-2
PH O'Farrell, High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry. ,vol. 250, pp. 4007- 4021 ,(1975) , 10.1016/S0021-9258(19)41496-8
JG Breman, A Egan, GT Keusch, The intolerable burden of malaria: a new look at the numbers. American Journal of Tropical Medicine and Hygiene. ,vol. 64, ,(2001) , 10.4269/AJTMH.2001.64.IV
L. Keith Henry, Sanjay Khare, Cagdas Son, V. V. Suresh Babu, Fred Naider, Jeffrey M. Becker, Identification of a Contact Region between the Tridecapeptide α-Factor Mating Pheromone of Saccharomyces cerevisiae and Its G Protein-Coupled Receptor by Photoaffinity Labeling† Biochemistry. ,vol. 41, pp. 6128- 6139 ,(2002) , 10.1021/BI015863Z
Deepa Jethwaney, Timothy Lepore, Saria Hassan, Kerrianne Mello, Radha Rangarajan, Willi Jahnen-Dechent, Dyann Wirth, Ali A. Sultan, Fetuin-A, a Hepatocyte-Specific Protein That Binds Plasmodium berghei Thrombospondin-Related Adhesive Protein: a Potential Role in Infectivity Infection and Immunity. ,vol. 73, pp. 5883- 5891 ,(2005) , 10.1128/IAI.73.9.5883-5891.2005
T. Ifediba, J. P. Vanderberg, Complete in vitro maturation of Plasmodium falciparum gametocytes Nature. ,vol. 294, pp. 364- 366 ,(1981) , 10.1038/294364A0
MC Rodriguez, Margos G, Compton H, Ku M, Lanz H, Plasmodium berghei: routine production of pure gametocytes, extracellular gametes, zygotes, and ookinetes. Experimental Parasitology. ,vol. 101, pp. 73- 76 ,(2002) , 10.1016/S0014-4894(02)00035-8
Rita Tewari, Roberta Spaccapelo, Francesco Bistoni, Anthony A. Holder, Andrea Crisanti, Function of region I and II adhesive motifs of Plasmodium falciparum circumsporozoite protein in sporozoite motility and infectivity. Journal of Biological Chemistry. ,vol. 277, pp. 47613- 47618 ,(2002) , 10.1074/JBC.M208453200
David N. Perkins, Darryl J. C. Pappin, David M. Creasy, John S. Cottrell, Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis. ,vol. 20, pp. 3551- 3567 ,(1999) , 10.1002/(SICI)1522-2683(19991201)20:18<3551::AID-ELPS3551>3.0.CO;2-2