Fluorescence correlations, single molecule detection and large number screening Applications in biotechnology

摘要: Fluorescence correlation spectroscopy (FCS), when carried out under conditions with low background as obtained in very small volume elements, is a powerful tool for examining molecular interactions well their time dependence. Interactions of biological importance which can be analyzed are hybridization between nucleic acid primers and DNA or RNA targets, peptide ligands isolated cell-bound receptors, antigen antibodies. Since the interaction rapidly volumes without need separating unbound from bound ligand, an important application FCS envisaged large-scale drug screening. The sensitivity has been advanced to point that detection single dye molecules possible submillisecond range. This opens up possibility detecting rare events such appearance pathogens early phase infection mutants exhibiting unusual properties screening combinatorial libraries.