FCS-Analysis of Ligand-Receptor Interactions in Living Cells
作者： Alladin Pramanik , Rudolf Rigler
DOI: 10.1007/978-3-642-59542-4_6
关键词: Fluorescence correlation spectroscopy 、 Chemistry 、 Biophysics 、 Confocal 、 Receptor 、 Cellular level 、 Resolution (electron density) 、 Ligand (biochemistry)
摘要: Fluorescence correlation spectroscopy (FCS) in its present form using confocal llumination volumes with single molecule detection sensitivity [6.1,6.2] appears as a foremost tool for the analysis of dynamic processes on cell surfaces well interior cells. FCS used after introduction 1970s [6.3-6.5] involved large excitation and correspondingly long times. In contrast to today, was prone photodestruction, successful applications at cellular level were difficult or even impossible. Submicrometer resolution provided by setup now allows detailed defined localization within has reopened era quantitative biology which once pioneered Torbjorn Caspersson Karolinska Institute his seminal work UV microspectroscopy [6.6].
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