Photoaffinity labeling of NAD(+)-linked enzymes.

摘要: Publisher Summary Nicotinamide adenine dinucleotide (NAD + ) is involved in numerous enzyme-catalyzed reactions that are important intermediary metabolism, DNA repair, and signal transduction. In these reactions, NAD functions either as a redox coenzyme or substrate for mono- poly(ADP)-ribosylation. may also serve precursor cyclic ADP-ribose, potential second messenger calcium mobilization. Photoaffinity labeling using photoaffinity probes has been shown to be an effective technique characterizing the nucleotide-binding properties of number proteins. A probes, which differ structural location photoactivatible group, introduced chapter. These inert analogs form highly reactive intermediates on activation by exposure light proper wavelength, usually ultraviolet (UV) range. Nucleotides possessing photoreactive group attached base most commonly used this type. Several have designed -linked enzymes. include 32 P-labeled 2N 3 -NAD ( [ P]2N ), 125 I-labeled N 6 -substituted azido analog , carbonyl -C]NAD.