Strategies and reagents for photoaffinity labeling of mechanochemical proteins.

摘要: Publisher Summary This chapter presents some of the uses nucleotide photoaffinity probes to study various aspects interaction nucleotides with mechanochemical proteins. These applications range from detecting subunits involved in binding isolation and sequencing active site peptides that are photolabeled. In general, have distinct advantage over classic chemical photoactive group is not chemically reactive without exposure proper wavelength light. Several photoattinity proved be excellent tools for identifying protein bind specific even when studying crude homogenates. They also quite effective at determining size approximate location within under conditions where large enough separated by SDS-PAGE. However, a number problems been encountered, which particularly serious attempting define region higher resolution. To resolve HPLC analysis photolabeled peptides, an alternative approaches purifying peptide, using only confirm purity peptide isolated resorted to. A good general technique anion-exclusion chromatography, along approach validate labeling, presented chapter.