Viscoelastic properties of tubule basement membranes in experimental renal cystic disease

摘要: Viscoelastic properties of tubule basement membranes in experimental renal cystic disease. Hereditary and acquired cysts develop from segments that enlarge progressively. We measured the deformability surrounding individual normal tubules to determine if might by simple extension abnormally-deformable membrane response or increased transtubule hydrostatic pressures. Deformability (cm/dyne) was vitro a micropipet aspiration technique related negative pressures within pipet distance cyst wall aspirated into pipet. creep determined time-dependent effect on deformation. Proximal collecting tubules, glomerular capsules were microdissected controls animals with (Diphenylthiazole [rats], Nordihy-droguaiaretic acid [rats]), hereditary (C57 BL/6J [cpk/cpk] mice) spontaneous (CFW w diseases. The major resistance deformation localized since collagenase destroyed elasticity walls. Tubule adjacent appeared abnormal electron microscopy fed DPT, but measurements viscoelastic showed no differences between any animal model. values (7.7 ± 1.1, 10.9 11.2 0.6, 9.4 0.8 × 10 -3 cm/dyne NDGA, C57 CF , respectively) are consistent interpretation high ranging 39 134 cm H 2 0 would be required form stretching secondary transepithelial pressure-gradient. Since vivo across walls not enough we conclude do due membranes.