Dengue virus strain 2 capsid protein switches the annealing pathway and reduces the intrinsic dynamics of the conserved 5' untranslated region

摘要: ABSTRACT The capsid protein of Dengue Virus strain 2 (DENV2C) is a structural with RNA chaperone activity that promotes multiple nucleic acid rearrangements, critical for transcription the single-stranded positive-sense DENV2 genomic RNA. Annealing conserved 5’ untranslated region (5’UTR) to either its complementary sequence or 3’ (3’UTR) occurs during (+)/(−) ds-RNA formation and (+) circularization, respectively, both essential steps DENV replication. We investigated effect DENV2C on annealing mechanism two hairpin structures from 5’UTR (21-nt upstream AUG (5’UAR) 23-nt capsid-coding (5’cHP)) their sequences circularization. Using fluorescence spectroscopy, was found switch reactions nucleated mainly through kissing-loop intermediates stem-stem interactions while it FRET-FCS trFRET, we determined exerts activities by modulating intrinsic dynamics reducing kinetically trapped unfavorable conformations sequence. Thus, likely facilitate genome folding into functional required replication, playing role in