RNA Sequences and Structures Required for the Recruitment and Activity of the Dengue Virus Polymerase

作者: Claudia V. Filomatori , Nestor G. Iglesias , Sergio M. Villordo , Diego E. Alvarez , Andrea V. Gamarnik

DOI: 10.1074/JBC.M110.162289

关键词:

摘要: Dengue virus RNA-dependent RNA polymerase specifically binds to the viral genome by interacting with a promoter element known as stem-loop A (SLA). Although great deal has been learned in recent years about function of this dengue virus-infected cells, molecular details that explain how SLA interacts promote synthesis remain poorly understood. Using binding and activity assays, we defined two elements are involved interaction synthesis. Mutations at top resulted RNAs retained ability bind but impaired promoter-dependent These results indicate protein is not sufficient induce specific nucleotides necessary render an active polymerase-promoter complex for We also report induces conformational changes downstream element. Furthermore, found structured 3′ end template repress context fully promoter. assays evaluate initiation 3′-UTR, RNA-RNA mediated 5′-3′-hybridization was able release silencing effect 3′-stem-loop structure. propose long range interactions play multiple roles during Together, provide new activity.

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