Abnormal Na+/H+ antiporter phenotype and turnover of immortalized lymphoblasts from type 1 diabetic patients with nephropathy.

摘要: Cellular Na+/H+ exchanger (NHE) activity is elevated in type 1 diabetic patients with nephropathy and essential hypertension. The characteristics of this NHE phenotype hypertension (raised Vmax a lowered Hill coefficient) are preserved Epstein-Barr virus-transformed lymphoblasts from hypertensive patients. In study, we have determined kinetics cultured without nephropathy, nondiabetic controls, using fluorometry the pH indicator 2,7'-bis-(carboxyethyl)-5,6-carboxyfluorescein estimation isoform (NHE-1) density specific polyclonal antibodies. was significantly, coefficient for internal H+ binding cells compared both normal controls normoalbuminuric NHE-1 as measured by Western blotting similar all groups. turnover number thus This resembles that suggests such may predisposition to Moreover, these changes persist vitro, should provide cell culture model further define basic mechanisms leading activation nephropathy.