Sorting and secretion of adrenocorticotropin in a pituitary tumor cell line after perturbation of the level of a secretory granule-specific proteoglycan.

作者: T L Burgess , R B Kelly

DOI: 10.1083/JCB.99.6.2223

关键词: ProteoglycanSecretionBiochemistryChondroitin sulfateAnterior pituitaryCell cultureBiologyPituitary neoplasmCell fractionationXyloside

摘要: A mouse anterior pituitary tumor cell line (AtT-20) that secretes adrenocorticotropin and beta endorphin sorts the proteins it transports to surface into two exocytotic pathways. AtT-20 cells also synthesize a secretory granule-specific sulfated molecule secrete on stimulation (Moore, H.-P., B. Gumbiner, R. Kelly, 1983, J. Cell Biol., 97:810-817). We show here this is sensitive proteolysis residual material co-migrates with chondroitin sulfate standard thin-layer electrophoresis. Furthermore, completely chondroitinase ABC digestion. Thus proteoglycan side chains. examined role of proteoglycans in sorting secretion by severely decreasing amount vesicle-specific ways. First, xyloside was used inhibit biosynthesis; second, variant isolated synthesized little proteoglycan. In neither case or detectably altered, suggesting not required for these processes.

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