Response to oxidative stress induced by cadmium and copper in tobacco plants (Nicotiana tabacum) engineered with the trehalose-6-phosphate synthase gene (AtTPS1)

摘要: The response of tobacco plants genetically engineered with the AtTPS1 gene to stress induced by excess Cu and Cd was evaluated in hydroponic solution (100 400 lM 50 200 Cd) after a 48 h exposure. Two transgenic lines, transformed (trehalose-6-phosphate synthase) from Arabidopsis, different levels trehalose-6-phosphate synthase expression (B5H, higher B1F, lower), wild type (WT) were investigated. Protein content, anti- oxidative enzymes (CAT, POD, SOD, APX), glucose, fructose, lipid peroxidation, hydrogen peroxide contents determined leaves. two lines differently influenced exposure as they antioxidant enzymatic defense response. B1F B5H showed better acclimation compared WT. Furthermore more tolerant than Cu. accumulated less leaves, probably due efficient exclusion mechanism. Catalase shown be most important enzyme antioxidative sys- tem these plants.