Selected-ion monitoring gas chromatographic—mass spectrometric analysis of catecholamines: enhancement of sensitivity by a simple clean-up step on Sephadex G-10
作者： Gerhard K.E. Scriba , Ronald T. Borchardt , Joseph A. Zirrolli , Paul V. Fennessey
DOI: 10.1016/S0378-4347(00)80582-4
关键词: Electron ionization 、 Selected ion monitoring 、 Sephadex 、 Detection limit 、 Gas chromatography 、 Mass spectrometry 、 Chromatography 、 Chemistry 、 High-performance liquid chromatography 、 Gas chromatography–mass spectrometry
摘要: The sensitivity of catecholamines using selected-ion monitoring gas chromatography-mass spectrometry (SIM GC-MS) has been enhanced by employing a simple clean-up step Sephadex G-10 columns. This procedure allows for the detection extremely small amounts (1-10 pg) biogenic amines (e.g., epinine) which corresponds to 25-fold increase in limits compared electron ionization GC-MS or high-performance liquid chromatography with electrochemical (HPLC-ED). SIM assay was used monitor dopamine-beta-hydroxylase-mediated conversion epinine d6 epinephrine rat hypothalamus and brainstem vitro results were those obtained HPLC-ED.
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