Dose-Dependent Cannabidiol-Induced Elevation of Intracellular Calcium and Apoptosis in Human Articular Chondrocytes.

摘要: Cannabidiol (CBD) is the most abundant non-psychoactive compound of Cannabis sativa extracts. Cannabinoids have been shown to exhibit anti-inflammatory, analgesic, antioxidant, neuroprotective, and anti-tumorigenic effects. In present study, we investigated effects CBD on human articular chondrocytes. Cell viability was determined by Resazurin assays. Apoptosis analyzed annexin-V/7-actinomycin D (7-AAD) staining followed flow cytometry. Caspase 3/7 activity measured with caspase Intracellular Ca2+ ([Ca2+ ]i ) monitored time-lapse fluorescence imaging. The perforated whole-cell patch-clamp technique used for measuring cell membrane potential. Erk1/2 phosphorylation assessed western blot analysis. chondrocyte line C28/I2 primary chondrocytes showed a reduced after treatment concentrations greater than 4 µM. This apoptotic effect accompanied an increase in early population. elevated [Ca2+ , which depolarization abrogated, when omitted from bath solution, indicating influx extracellular . cannabinoid receptor 1 (CB1) antagonist AM251 inhibited triggered CBD. Preincubation toxic By looking mediators downstream CB1 receptor, enhanced could be detected treatment. However, this activation proved unaffected blockage. study demonstrates that promotes apoptosis elevation via CB1-receptor-mediated mechanism. © 2019 Authors. Journal Orthopaedic Research® published Wiley Periodicals, Inc. behalf Research Society J Orthop Res 37:2540-2549, 2019.