Process for isolating albumin from blood

作者: Christian Frohlich , Waldemar Schneider , Hans Lefevre , Harald Fiedler

DOI:

关键词: Blood plasmaAliphatic alcoholVolume concentrationChromatographyAlbuminAlcoholSerum albuminChemistry

摘要: Method for the abstraction of pure serum albumin from blood which includes steps separating plasma solid constituents and isolating dissolved non-albumin plasma, adding an albumin-stabilizer treating such fluid with a lower aliphatic alcohol, whereby, treatment is carried out at volume concentration alcohol 7 to 14% in presence 0.001 0.1 moles stabilizer, temperature 60° 75° C. pH 4.5 7.5; concomitant proteins, partly or largely denatured, are precipitated; resultant solution containing separated precipitate 1° 30° C., preferably room temperature.

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