作者: Christian Frohlich , Waldemar Schneider , Hans Lefevre , Harald Fiedler
DOI:
关键词: Blood plasma 、 Aliphatic alcohol 、 Volume concentration 、 Chromatography 、 Albumin 、 Alcohol 、 Serum albumin 、 Chemistry
摘要: Method for the abstraction of pure serum albumin from blood which includes steps separating plasma solid constituents and isolating dissolved non-albumin plasma, adding an albumin-stabilizer treating such fluid with a lower aliphatic alcohol, whereby, treatment is carried out at volume concentration alcohol 7 to 14% in presence 0.001 0.1 moles stabilizer, temperature 60° 75° C. pH 4.5 7.5; concomitant proteins, partly or largely denatured, are precipitated; resultant solution containing separated precipitate 1° 30° C., preferably room temperature.