Fabrication of Tapered Microtube Arrays and Their Application as a Microalgal Injection Platform
作者： Andrew R. Durney , Leah C. Frenette , Elizabeth C. Hodvedt , Todd D. Krauss , Hitomi Mukaibo
关键词: Atomic layer deposition 、 Materials science 、 Membrane 、 Quantum dot 、 Optoelectronics 、 Electrical conductor 、 Nanotechnology 、 Fabrication 、 Pulse duration 、 Successful injection 、 Intact cell
摘要: A template-synthesis method that enables fabrication of tapered microtube arrays is reported. Track-etched poly(ethylene terephthalate) membranes are used as the template, with closed-tipped conical pores having length and base diameter 6.27 ± 0.28 1.21 0.05 μm, respectively. conductive layer Pt deposited by atomic deposition (ALD) to enable successive electrodeposition Ni. By decreasing precursor pulse duration from 10 1 s during ALD step, heights microtubes controlled maximal full (∼6 μm) only a fraction (1-2 template pore. Using pulsed-current (PCD) method, smooth uniform Ni deposit achieved thickness can be function PCD cycle. The microtubes' lumen confirmed stay open even after 2000 cycles PCD. potential application prepared array microinjection platform demonstrated via successful injection nm sized CdZnS/ZnS core/shell quantum dots into Chlamydomonas reinhardtii microalgae cells intact cell walls. direct delivery in this paper offers novel opportunities for extending growing interest array-based microalgal systems.
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