作者: Irene M. Unger , Keith W. Goyne , Ann C. Kennedy , Robert J. Kremer , Jean E.T. McLain
关键词: Fluorescein diacetate hydrolysis 、 Soil water 、 Microbial population biology 、 Community structure 、 Lincomycin 、 Manure 、 Mesocosm 、 Agroecosystem 、 Agronomy 、 Biology 、 Ecology
摘要: Veterinary antibiotics (VAs) administered to livestock are introduced agroecosystems via land application of manure, posing a potential human and environmental health risk. Recent evidence suggests that agroforestry grass vegetative filter strips (VFS) may act mitigate VA transport or enhance degradation; however, VAs adversely affect soil microbial communities within VFS thus alter the primary functioning VFS. The objectives this research were investigate changes in community structure function quantify development antibiotic resistance no-till soils exposed various classes concentrations. Laboratory mesocosms established using collected from cropland two (grass agroforestry). Soils treated with oxytetracycline lincomycin (5–200 mg kg⁻¹ soil). Individual underwent destructive sampling at nine time points during 63 d, tested for (C-utilization, dehydrogenase, fluorescein diacetate hydrolysis assays), (phospholipid fatty acid analysis), resistance. Functional assays associated all treatments showed an initial inhibitory effect, but trend was generally reversed by seventh day. Shifts increased not observed, suggesting robust effects test This work indicates loss will diminish important functions use agriculture.