Selection of stem cell clones with defined differentiation capabilities
作者： Olivier Preynat-Seauve , Karl-Heinz Krause
DOI:
关键词: Biology 、 Screening test 、 Genetics 、 In vitro 、 Cell biology 、 Selection (genetic algorithm) 、 Homogeneous 、 Population 、 Stem cell 、 Neural cell
摘要: Disclosed are methods for producing a clonal population of cells involving: a) obtaining pluripotent or multipotent that have been expanded in vitro and maintained an undifferentiated essentially state; b) expanding individualized the into populations cells; c) selecting one more determined to ability differentiate is at least about 50% homogeneous either neural cell types, hepatocytes, cardiomyocytes. Also disclosed produced by present invention, treating disease subjects involving administration invention subject. Methods screening test compounds involve contacting compound with also set forth.
lens.org 参考文章(59)
Qi-Long Ying, Jennifer Nichols, Ian Chambers, Austin Smith, BMP Induction of Id Proteins Suppresses Differentiation and Sustains Embryonic Stem Cell Self-Renewal in Collaboration with STAT3 Cell. ,vol. 115, pp. 281- 292 ,(2003) , 10.1016/S0092-8674(03)00847-X
Y. Toyooka, D. Shimosato, K. Murakami, K. Takahashi, H. Niwa, Identification and characterization of subpopulations in undifferentiated ES cell culture Development. ,vol. 135, pp. 909- 918 ,(2008) , 10.1242/DEV.017400
M. S. Cho, Y.-E. Lee, J. Y. Kim, S. Chung, Y. H. Cho, D.-S. Kim, S.-M. Kang, H. Lee, M.-H. Kim, J.-H. Kim, J. W. Leem, S. K. Oh, Y. M. Choi, D.-Y. Hwang, J. W. Chang, D.-W. Kim, Highly efficient and large-scale generation of functional dopamine neurons from human embryonic stem cells. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 105, pp. 3392- 3397 ,(2008) , 10.1073/PNAS.0712359105
Lesley Gerrard, Debiao Zhao, A. John Clark, Wei Cui, Stably transfected human embryonic stem cell clones express OCT4-specific green fluorescent protein and maintain self-renewal and pluripotency Stem Cells. ,vol. 23, pp. 124- 133 ,(2005) , 10.1634/STEMCELLS.2004-0102
Shobha Mareddy, Navdeep Dhaliwal, Ross Crawford, Yin Xiao, Stem cell-related gene expression in clonal populations of mesenchymal stromal cells from bone marrow. Tissue Engineering Part A. ,vol. 16, pp. 749- 758 ,(2010) , 10.1089/TEN.TEA.2009.0307
Anand Srivastava, Rakesh Malhotra, Jason Sharp, Travis Berggren, Potentials of ES Cell Therapy in Neurodegenerative Diseases Current Pharmaceutical Design. ,vol. 14, pp. 3873- 3879 ,(2008) , 10.2174/138161208786898617
Kouichi Hasegawa, Tsuyoshi Fujioka, Yukio Nakamura, Norio Nakatsuji, Hirofumi Suemori, A Method for the Selection of Human Embryonic Stem Cell Sublines with High Replating Efficiency After Single‐Cell Dissociation Stem Cells. ,vol. 24, pp. 2649- 2660 ,(2006) , 10.1634/STEMCELLS.2005-0657
J. Yu, J. A. Thomson, Pluripotent stem cell lines Genes & Development. ,vol. 22, pp. 1987- 1997 ,(2008) , 10.1101/GAD.1689808
Amar M. Singh, Takashi Hamazaki, Katherine E. Hankowski, Naohiro Terada, A Heterogeneous Expression Pattern for Nanog in Embryonic Stem Cells Stem Cells. ,vol. 25, pp. 2534- 2542 ,(2007) , 10.1634/STEMCELLS.2007-0126
Morag H Stewart, Marc Bossé, Kristin Chadwick, Pablo Menendez, Sean C Bendall, Mickie Bhatia, Clonal isolation of hESCs reveals heterogeneity within the pluripotent stem cell compartment Nature Methods. ,vol. 3, pp. 807- 815 ,(2006) , 10.1038/NMETH939