A murine model of bone marrow micrometastasis in breast cancer.
作者： Barbara O'Kane Murphy , Shantaram Joshi , Anne Kessinger , Elizabeth Reed , J. Graham Sharp
关键词: Hematology 、 Biology 、 Clone (cell biology) 、 Micrometastasis 、 Bone marrow 、 Internal medicine 、 Metastasis 、 Cell culture 、 Pathology 、 Mammary tumor 、 Breast cancer
摘要: Bone marrow (BM) is one of the most common sites and often first clinical indication metastatic progression breast cancer. Multivariate analyses have shown that presence cytokeratin positive tumor cells in women with newly diagnosed stage I, II or III cancer an independent predictor survival. The objective this study was to develop orthotopic model spontaneous BM metastasis facilitate studies process. A murine mammary adenocarcinoma cell line, Clone 66, transduced neomycin resistance gene (Cl66neo) injected orthotopically into female Balb/c mice. Polymerase chain reaction (PCR) for neo performed on harvested from bearing mice demonstrated as few 102 produced micrometastases at 4 weeks post-injection. Small foci were identified fatpad (mfp) without gross evidence primary tumors. Higher doses micrometastases, detectable by PCR, week Constructs containing green fluorescent protein (GFP) (neo) also 66 (Cl66-GFPneo) mfp. GFP multiple tissues addition flow cytometric analysis (FACS) but less 13% animals developed metastases. This a clinically relevant tool organ specificity metastasis.
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