PICK1 interacts with and regulates PKC phosphorylation of mGLUR7.
作者： Kumlesh K. Dev , Yoshiaki Nakajima , Jun Kitano , Steven P. Braithwaite , Jeremy M. Henley
DOI: 10.1523/JNEUROSCI.20-19-07252.2000
关键词: Metabotropic glutamate receptor 6 、 Molecular biology 、 Metabotropic glutamate receptor 3 、 Metabotropic glutamate receptor 4 、 Metabotropic glutamate receptor 、 Biology 、 Metabotropic glutamate receptor 1 、 Metabotropic glutamate receptor 2 、 Protein kinase C 、 Cell biology 、 Metabotropic glutamate receptor 7
摘要: The G-protein-coupled metabotropic glutamate receptor subtype 7a (mGluR7a) is a member of group III receptors that plays an important role as presynaptic in regulating transmitter release at glutamatergic synapses. Here we report the protein interacting with C-kinase (PICK1) binds to C terminus (ct) mGluR7a. In yeast two-hybrid system, extreme ct mGluR7a was shown interact PSD-95/Discs large/ZO-1 (PDZ) domain PICK1. Pull-down assays indicated PICK1 retained by glutathione S -transferase fusion ct-mGluR7a. Furthermore, recombinant and native PICK1/mGluR7a complexes were coimmunoprecipitated from COS-7 cells rat brain tissue, respectively. Confocal microscopy showed both displayed synaptic colocalization cultured hippocampal neurons. has previously been bind kinase α-subunit (PKCα), known be phosphorylated PKC. We show relationship between these three proteins using PICK1, mGluR7, PKCα, where they co-immunoprecipitated complex cells. addition, caused reduction PKCα-evoked phosphorylation vitro assays. These results suggest for modulating
bristol.ac.uk
sci-hub.se 参考文章(37)
S. K. Morris, W. P. C. Stemmer, Enzymatic inverse PCR: a restriction site independent, single-fragment method for high-efficiency, site-directed mutagenesis. BioTechniques. ,vol. 13, pp. 214- 220 ,(1992)
H.-C. Kornau, P. H. Seeburg, M. B. Kennedy, The Synaptic Protein Network Associated with Ionotropic Glutamate Receptors Handbook of experimental pharmacology. ,vol. 141, pp. 121- 142 ,(1999) , 10.1007/978-3-662-08022-1_3
N. Okamoto, S. Hori, C. Akazawa, Y. Hayashi, R. Shigemoto, N. Mizuno, S. Nakanishi, Molecular characterization of a new metabotropic glutamate receptor mGluR7 coupled to inhibitory cyclic AMP signal transduction. Journal of Biological Chemistry. ,vol. 269, pp. 1231- 1236 ,(1994) , 10.1016/S0021-9258(17)42247-2
Ryuichi Shigemoto, Ayae Kinoshita, Eiki Wada, Sakashi Nomura, Hitoshi Ohishi, Masahiko Takada, Peter J. Flor, Akio Neki, Takaaki Abe, Shigetada Nakanishi, Noboru Mizuno, Differential presynaptic localization of metabotropic glutamate receptor subtypes in the rat hippocampus. The Journal of Neuroscience. ,vol. 17, pp. 7503- 7522 ,(1997) , 10.1523/JNEUROSCI.17-19-07503.1997
Miwako Masugi, Mineto Yokoi, Ryuichi Shigemoto, Keiko Muguruma, Yasuyoshi Watanabe, Gilles Sansig, Herman van der Putten, Shigetada Nakanishi, Metabotropic Glutamate Receptor Subtype 7 Ablation Causes Deficit in Fear Response and Conditioned Taste Aversion The Journal of Neuroscience. ,vol. 19, pp. 955- 963 ,(1999) , 10.1523/JNEUROSCI.19-03-00955.1999
T.J. Bushell, G. Sansig, R. Shigemoto, P. Flor, R. Kuhn, Th. Knoepfel, M. Schroeder, V.L. Collett, G.L. Collingridge, H. van der Putten, An impairment of hippocampal synaptic plasticity in mice lacking mGlu7 receptors Neuropharmacology. ,vol. 35, ,(1996) , 10.1016/0028-3908(96)84669-7
Kumlesh K. Dev, Atsushi Nishimune, Jeremy M. Henley, Shigetada Nakanishi, The protein kinase Cα binding protein PICK1 interacts with short but not long form alternative splice variants of AMPA receptor subunits Neuropharmacology. ,vol. 38, pp. 635- 644 ,(1999) , 10.1016/S0028-3908(98)00230-5
Ayae Kinoshita, Ryuichi Shigemoto, Hitoshi Ohishi, Herman van der Putten, Noboru Mizuno, Immunohistochemical localization of metabotropic glutamate receptors, mGluR7a and mGluR7b, in the central nervous system of the adult rat and mouse: a light and electron microscopic study. The Journal of Comparative Neurology. ,vol. 393, pp. 332- 352 ,(1998) , 10.1002/(SICI)1096-9861(19980413)393:3<332::AID-CNE6>3.0.CO;2-2
Ryu Takeya, Koichiro Takeshige, Hideki Sumimoto, Interaction of the PDZ Domain of Human PICK1 with Class I ADP-Ribosylation Factors Biochemical and Biophysical Research Communications. ,vol. 267, pp. 149- 155 ,(2000) , 10.1006/BBRC.1999.1932
Z Songyang, AS Fanning, C Fu, J Xu, SM Marfatia, AH Chishti, A Crompton, AC Chan, JM Anderson, LC Cantley, Recognition of unique carboxyl-terminal motifs by distinct PDZ domains Science. ,vol. 275, pp. 73- 77 ,(1997) , 10.1126/SCIENCE.275.5296.73