Development of a quantitative chromatographic method for the determination of Imatinib and its main metabolite in human plasma

摘要: The objective of this master thesis was to develop an analytical method for the quantification cancer drug Imatinib and its main metabolite CGP74588 in plasma. is used treatment chronic myeloid leukemia gastrointestinal stroma tumors. A quantitative developed where reversed-phase columns with different stationary phases were studied sensitivity tested both UV detectors a mass spectrometric detection. Since substances measured plasma solid-phase extraction purify samples before analysis. column chosen separation Max-RP C12 (100 x 3 mm, 4 μm particle size) manufactured by Phenomenex gradient mobile phase 1% formic acid methanol water. as follows; 0 min 15:85, 7 60:40, 9 60:40 total runtime 13.5 min. internal standard Opipramol. Mass detection using sonic spray ionization interface positive mode proved be about sensitive at 261 nm. generated (M+H+)+ ions isolated fragmented use three methods; one (transition 494 —› 394), 480 394) Opipramol 364 171). For purification Oasis HLB cartridge selected recoveries close 100%. partially validated showed coefficients variation (CV) intra-and inter-day precision between 0.4 5.4% validation results spectrometer inconclusive.