In vivo imaging of free radicals: Applications from mouse to man

摘要: Free radicals and other paramagnetic species, play an important role in cellular injury pathophysiology. EPR spectroscopy imaging has emerged as tool for non-invasivein vivomeasurement spatial mapping of free biological tissues. Extensive applications have been performed small animals such mice recently humans performed. Spatial enables 3D the distribution a given radical while spectral-spatial spectral information at each position, and, from observed line width, localized tissue oxygenation can be determined. A variety spatial, These techniques, along with use biocompatible probes including particulate suspensions soluble nitroxide radicals, enable redox state biomedical applications. With imaging, was mapped within gastrointestinal (GI) tract living mice, enabling measurement oxygen gradient proximal to distal GI tract. Using metabolism major organs body visualized anatomically co-registered by proton MRI enablingin vivomapping clearance. techniques also applied non-invasively measure topically humans, providing regarding penetration label through skin its Thus, provided ranging animal models disease topical humans. (Mol Cell Biochem 234/235: 359-367, 2002)