Chemical-Chemical Combinations Map Uncharted Interactions in Escherichia coli under Nutrient Stress

摘要: Summary Of the ∼4,400 genes that constitute Escherichia coli 's genome, ∼300 are indispensable for its growth in nutrient-rich conditions. These encode housekeeping functions, including cell wall, DNA, RNA, and protein syntheses. Under conditions which nutrients limited to a carbon source, nitrogen essential phosphates, salts, more than 100 additional become essential. largely code synthesis of amino acids, vitamins, nucleobases. Although much is known about this collection ∼400 genes, their interactions under nutrient stress uncharted. Using chemical biology approach, we focused on 45 probes targeting encoded proteins mapped nutrient-limited Encompassing 990 unique pairwise combinations, revealed highly connected network 186 interactions, 81 were synergistic 105 antagonistic. The signature each probe highlighted new connectivity between functions those stress.