Comparison of enzymatic characterization and gene organization of cyclic nucleotide phosphodiesterase 8 family in humans
作者： Michiko Gamanuma , Keizo Yuasa , Takashi Sasaki , Naoki Sakurai , Jun Kotera
DOI: 10.1016/S0898-6568(02)00146-8
关键词: Biochemistry 、 Gene 、 Exon 、 Phosphodiesterase 、 Recombinant DNA 、 Biology 、 COS cells 、 Cyclic nucleotide phosphodiesterase 、 EHNA 、 PDE8B Gene 、 Molecular biology
摘要: Abstract Full-length cDNAs of human cyclic nucleotide phosphodiesterase 8B (PDE8B) were isolated. Enzymatic characteristics a dominant variant encoding protein 885 residues (PDE8B1) compared with those PDE8A1. The recombinant PDE8A1 and PDE8B1 proteins an entire form produced in both cytosolic membrane fractions the transfected COS cells. was high-affinity cAMP-PDE K m value 101±12 nM for cAMP, which is greater than that (40±1 nM). Relative V max 57±8% (100±12%). Although moderately inhibited by dipyridamole IC 50 8±2 μM, compound antagonized activity at three-fold higher concentration (IC =23±2 μM). PDE8B gene composed 22 exons, spanning over 217 kb. overall sequence identity between 68%, positions junctions each exon sequences well matched, indicating evolutionary relatedness genes.
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