Comparison between the matrices alpha-cyano-4-hydroxycinnamic acid and 4-chloro-alpha-cyanocinnamic acid for trypsin, chymotrypsin, and pepsin digestions by MALDI-TOF mass spectrometry.
作者： Thorsten W. Jaskolla , Dimitrios G. Papasotiriou , Michael Karas
DOI: 10.1021/PR900274S
关键词: Pepsin 、 Chymotrypsin 、 alpha-Cyano-4-hydroxycinnamic acid 、 Peptide 、 Chromatography 、 Chemistry 、 Protein mass spectrometry 、 Protease 、 Isoelectric point 、 Trypsin
摘要: The performance of the recently developed 4-chloro-α-cyanocinnamic acid (Cl-CCA) matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) matrix was investigated in comparison to most widely used α-cyano-4-hydroxycinnamic (CHCA). For this purpose, in-solution digestions standard proteins low femtomole range with proteases trypsin, chymotrypsin, and pepsin were as analytes. all protein−protease combinations, Cl-CCA revealed be highly superior terms number identified peptides, obtained sequence coverages peptide detection reproducibility. A deeper inspection detected signals regard both physicochemical properties (their isoelectric point) spectrometric (signal-to-noise ratios accuracies) showed that progress achieved is due numerous acidic neutral peptides. Moreover, higher sensitivity allowed for addit...
acs.org
core.ac.uk 
sci-hub.se 参考文章(28)
THE MULTIPLE SPECIFICITY OF CHYMOTRYPSIN Journal of Biological Chemistry. ,vol. 145, pp. 253- 265 ,(1942) , 10.1016/S0021-9258(18)45029-6
Joseph S. Fruton, Specificity of chymotrypsin B. Journal of Biological Chemistry. ,vol. 173, pp. 109- 110 ,(1948) , 10.1016/S0021-9258(18)35560-1
Kevin K Anderson, Matthew E Monroe, Don S Daly, Estimating probabilities of peptide database identifications to LC-FTICR-MS observations Proteome Science. ,vol. 4, pp. 1- 7 ,(2006) , 10.1186/1477-5956-4-1
Frank Fischer, Ansgar Poetsch, Protein cleavage strategies for an improved analysis of the membrane proteome Proteome Science. ,vol. 4, pp. 2- 2 ,(2006) , 10.1186/1477-5956-4-2
Bernhard Spengler, Dieter Kirsch, Raimund Kaufmann, Ernst Jaeger, Peptide sequencing by matrix-assisted laser-desorption mass spectrometry Rapid Communications in Mass Spectrometry. ,vol. 6, pp. 105- 108 ,(1992) , 10.1002/RCM.1290060207
J. S. Fruton, M. Bergmann, THE SPECIFICITY OF PEPSIN ACTION. Science. ,vol. 87, pp. 557- 557 ,(1938) , 10.1126/SCIENCE.87.2268.557
Tao Liu, Wei-Jun Qian, Marina A. Gritsenko, David G. Camp, Matthew E. Monroe, Ronald J. Moore, Richard D. Smith, Human Plasma N-Glycoproteome Analysis by Immunoaffinity Subtraction, Hydrazide Chemistry, and Mass Spectrometry Journal of Proteome Research. ,vol. 4, pp. 2070- 2080 ,(2005) , 10.1021/PR0502065
Michael. Karas, Franz. Hillenkamp, Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons Analytical Chemistry. ,vol. 60, pp. 2299- 2301 ,(1988) , 10.1021/AC00171A028
Jack Kyte, Russell F. Doolittle, A simple method for displaying the hydropathic character of a protein Journal of Molecular Biology. ,vol. 157, pp. 105- 132 ,(1982) , 10.1016/0022-2836(82)90515-0
William J. Henzel, Colin Watanabe, John T. Stults, Protein identification: The origins of peptide mass fingerprinting Journal of the American Society for Mass Spectrometry. ,vol. 14, pp. 931- 942 ,(2003) , 10.1016/S1044-0305(03)00214-9