Evaluation of the New MALDI Matrix 4-Chloro-Alpha-Cyanocinnamic Acid

摘要: RP-53 Matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF) continues to be an important tool for many proteomic studies. Recently Jaskolla, et.al. introduced a new rationally designed matrix (4 chloro-alpha-cyanocinnamic acid) which is reported have superior performance as compared the “gold standard” alpha-cyano-4-hydroxycinnamic acid. We decided investigate this in Shimadzu Biotech Axima TOF2. In study we acid analysis protein digests. looked at overall sequence coverage well sensitivity comparison with standard tryptic also labile peptides such phosphopeptides and 4-sulphophenyl isothiocynate derivitzed (SPITC) facilitate de novo sequencing. This was found better performing than alpha cyano-4-hydroxycinnamic since it has less bias C-terminal arginine containing peptides. It showed much 5 fold improvement stainless steel targets. addition due cooler nature are readily seen intact fragmentation MS mode. evaluated MS/MS modes both post-source-decay (PSD) collisional induced dissociation (CID). that occurs CID however very cool PSD fragments quite weak. promises already extensive array MALDI matrices once good commercial source highly purified preparation becomes available. T.W.; Lehmann, W.; Karas, M. Proc. Natl. Acad. Sci. U.S.A. 2008, 105, 12200-12205.