GUMBOS matrices of variable hydrophobicity for matrix-assisted laser desorption/ionization mass spectrometry.

摘要: RATIONALE Detection of hydrophobic peptides remains a major obstacle for matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). This stems from the fact that most matrices MALDI are hydrophilic and therefore have low affinities peptides. Herein, 1-aminopyrene (AP) AP-derived group uniform materials based on organic salts (GUMBOS) as novel MALDI-MS analyses were investigated peptides. METHODS A number solid-phase AP-based GUMBOS synthesized with variable hydrophobicity simply by changing counterions. Structures confirmed use 1H NMR electrospray ionization (ESI-MS). 1-Octanol/water partition coefficients (Ko/w) used to measure matrices. A dried-droplet method was sample preparation. All spectra obtained using MALDI-TOF spectrometer in positive ion reflectron mode. RESULTS A series including [AP][chloride] ([AP][Cl]), [AP][ascorbate] ([AP][Asc]) [AP][bis(trifluoromethane)sulfonimide] ([AP][NTf2]). The relative hydrophobicities these compounds α-cyano-4-hydroxycinnamic acid (CHCA, common matrix) indicated can be tuned much more than CHCA. clear trend is observed between signal intensities matrix. CONCLUSIONS MALDI tunable easily varying counterion. We found matrix very effective determination and, similarly, displayed greater intensity matrix. Copyright © 2014 John Wiley & Sons, Ltd.