Tissue distribution of neutral deoxyribonuclease (DNase) activity in the mussel Mytilus galloprovincialis
作者： Nevenka Bihari , Maja Fafanđel , Lorena Perić
DOI: 10.1016/J.CBPB.2007.03.008
关键词: Molecular biology 、 Enzyme assay 、 Gill 、 Biology 、 Hepatopancreas 、 Mussel 、 Enzyme 、 Zymography 、 Mytilus 、 Deoxyribonuclease
摘要: The presence of neutral DNase activity in bivalves is reported for the first time. enzyme four tissues mussel Mytilus galloprovincialis was analyzed by three different methods (i) specific denaturating SDS-PAGE zymogram, (ii) sensitive single radial diffusion (SRED) assay and (iii) rapid fluorimetric determination with PicoGreen. enough hydrolytic dsDNA hepatopancreas, adductor, gills mantle. Maximal all tissue extracts obtained Ca2+ Mg2+ at pH 7.0 as substrate. decreases order mantle > adductor. displays interindividual variability particular well among within one specimen. In hepatopancreas to distinct proteins expressing neutral, Ca2+, Mg2+-dependent, were detected zymogram. This heterogeneity nucleases involved DNA hydrolysis could reflect food utilization nutrient requirement.
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