Genetic diversity of Francisella tularensis subsp. holarctica in Kazakhstan.

摘要: Tularemia is a highly dangerous zoonotic infection due to the bacteria Francisella tularensis. Low genetic diversity promoted use of polymorphic tandem repeats (MLVA) as first-line assay for description. Whole genome sequencing (WGS) becoming increasingly accessible, opening perspective time when WGS might become universal genotyping assay. The main goal this study was describe F. tularensis strains circulating in Kazakhstan based on data and develop MLVA compatible with vitro silico analysis. In were performed vaccine strain 38 isolated from natural water bodies, ticks, rodents, carnivores, one migratory bird, an Isabellina wheatear captured rodent burrow. two approaches congruent allowed attribute all holarctica lineages, B.4 B.12. seven investigated collection could be typed single multiplex PCR Identical genotypes produced by analysis, demonstrating full compatibility between approaches. compared publicly available worldwide origin whole SNP (wgSNP) Genotypes differing at position collected within interval more than fifty years, locations separated each other thousand kilometers, supporting role birds spread bacteria.