Identification of strain specific markers in Bacillus anthracis by random amplification of polymorphic DNA.
作者： Haim Levy , Morly Fisher , Naomi Ariel , Zeev Altboum , David Kobiler
DOI: 10.1016/J.FEMSLE.2005.01.039
关键词:
摘要: Classification and differentiation of Bacillus anthracis isolates by genetic markers play an important role in anthrax research. We used a PCR based method – Random Amplification Polymorphic DNA (RAPD) to identify B. strains. Twenty-five differential were identified which divided the strains into five different groups. Three selected RAPD-markers cloned sequenced. The RAPD-derived genotypes could be defined integration these three markers. This system offers simple non-expensive classify laboratories involved research this bacterium.
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