Studies on the formation of the initiation complex in eukaryotes.
作者： Stuart M. Heywood , William C. Thompson
DOI: 10.1016/0006-291X(71)90637-1
关键词: Eukaryotic Small Ribosomal Subunit 、 Biochemistry 、 GTP' 、 Prokaryotic initiation factor-2 、 eIF4A 、 Eukaryotic initiation factor 、 eIF2 、 Biology 、 Eukaryotic Ribosome 、 Initiation factor
摘要: Abstract The initial interaction between a natural messenger RNA and ribosomes in eukaryotic system was studied. Utilizing population of 1.0 M KCl-washed isolated myosin mRNA, only the 40S subunits bound mRNA to form initiation complex I. This binding required presence factor EF 3 either GTP or its analogue, GDPCP. When unfractionated factors, tRNA were added reaction mixture, 60S ribosomal subunit joined with I produce II. Complex II could not be demonstrated absence when replaced by
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sci-hub.se 参考文章(6)
Gunter von Ehrenstein, [76] Isolation of sRNA from intact Escherichia coli cells Methods in Enzymology. ,vol. 12, pp. 588- 596 ,(1967) , 10.1016/S0076-6879(67)12088-0
Stuart M. Heywood, Mark Nwagwu, Partial characterization of presumptive myosin messenger ribonucleic acid. Biochemistry. ,vol. 8, pp. 3839- 3845 ,(1969) , 10.1021/BI00837A050
David Kabat, Phosphorylation of ribosomal proteins in rabbit reticulocytes. Characterization and regulatory aspects Biochemistry. ,vol. 9, pp. 4160- 4175 ,(1970) , 10.1021/BI00823A019
STUART M. HEYWOOD, Formation of the Initiation Complex using Muscle Messenger RNAs Nature. ,vol. 225, pp. 696- 698 ,(1970) , 10.1038/225696A0
Ester A. Zylber, Sheldon Penman, The effect of high ionic strength on monomers, polyribosomes, and puromycin-treated polyribosomes. Biochimica et Biophysica Acta. ,vol. 204, pp. 221- 229 ,(1970) , 10.1016/0005-2787(70)90505-8
P Lengyel, D Söll, Mechanism of protein biosynthesis. Bacteriological Reviews. ,vol. 33, pp. 264- 301 ,(1969) , 10.1128/MMBR.33.2.264-301.1969