Age-dependent accumulation of Dolichol in Rat Liver: is tissue dolichol a Biomarker of Aging?
作者： M. Marino , C. Dolfi , C. Paradiso , G. Cavallini , M. Masini
关键词: Calorie restriction 、 Biology 、 Internal medicine 、 Dolichol 、 Parallel study 、 Endocrinology 、 Autophagy 、 Oxidative stress 、 Hepatocyte 、 Proteolysis 、 Biomarker (medicine)
摘要: Dolichols are long hydrophobic molecules broadly distributed in all tissues and cellular membranes of eukariotic cells. Dolichol affects membrane structure fluidity, membrane-associated protein activities, sensitivity to oxidative stress. Reports have shown that dolichols exhibit a remarkable (6- 30-fold) age-related increase the adult mature rats old flies, mice, humans. In our longitudinal study, accumulation dolichol was monitored liver tissue male Sprague Dawley fed ad libitum up age 27 months. addition 24-month-old subjected different regimens anti-aging diet restriction (40% calorie or every-other-day feeding libitum) were tested. A parallel study carbonyl (a proposed biomarker aging) made. addition, decline autophagy/proteolysis studied isolated cells, view essential role this function maintenance. Results show an age-dependent can be observed but not food-restricted rats, precedes altered proteins, is accompanied by autophagy. It concluded satisfies primary secondary applicable criteria desirable features required qualified as aging.
oup.com
oxfordjournals.org
sci-hub.se 参考文章(59)
L. F. Leloir, The Role of Dolichol in Protein Glycosylation Function and Biosynthesis of Lipids. ,vol. 83, pp. 9- 19 ,(1977) , 10.1007/978-1-4684-3276-3_3
T Chojnacki, G Dallner, The biological role of dolichol. Biochemical Journal. ,vol. 251, pp. 1- 9 ,(1988) , 10.1042/BJ2510001
G. Van Dessel, M. De Wolf, H. J. Hilderson, A. Lagrou, W. Dierick, Intracellular and Extracellular Flow of Dolichol Subcellular Biochemistry. ,vol. 16, pp. 227- 278 ,(1990) , 10.1007/978-1-4899-1621-1_8
Abraham Z. Reznick, Lester Packer, [38] Oxidative damage to proteins: Spectrophotometric method for carbonyl assay Methods in Enzymology. ,vol. 233, pp. 357- 363 ,(1994) , 10.1016/S0076-6879(94)33041-7
Per O. Seglen, Preparation of isolated rat liver cells. Methods in Cell Biology. ,vol. 13, pp. 29- 83 ,(1976) , 10.1016/S0091-679X(08)61797-5
W A Maltese, R A Erdman, Characterization of isoprenoid involved in the post-translational modification of mammalian cell proteins. Journal of Biological Chemistry. ,vol. 264, pp. 18168- 18172 ,(1989) , 10.1016/S0021-9258(19)84692-6
Kenneth H. Woodside, Glenn E. Mortimore, Suppression of Protein Turnover by Amino Acids in the Perfused Rat Liver Journal of Biological Chemistry. ,vol. 247, pp. 6474- 6481 ,(1972) , 10.1016/S0021-9258(19)44717-0
C Valtersson, G van Duÿn, A J Verkleij, T Chojnacki, B de Kruijff, G Dallner, The influence of dolichol, dolichol esters, and dolichyl phosphate on phospholipid polymorphism and fluidity in model membranes. Journal of Biological Chemistry. ,vol. 260, pp. 2742- 2751 ,(1985) , 10.1016/S0021-9258(18)89424-8
G Miotto, R Venerando, O Marin, N Siliprandi, G E Mortimore, INHIBITION OF MACROAUTOPHAGY AND PROTEOLYSIS IN THE ISOLATED RAT HEPATOCYTE BY A NONTRANSPORTABLE DERIVATIVE OF THE MULTIPLE ANTIGEN PEPTIDE LEU8-LYS4 -LYS2-LYS-BETA ALA Journal of Biological Chemistry. ,vol. 269, pp. 25348- 25353 ,(1994) , 10.1016/S0021-9258(18)47254-7
R K Pullarkat, P S Pullarkat, G N Morris, Phosphorylated dolichols in aging. Biochemical Journal. ,vol. 265, pp. 891- 894 ,(1990) , 10.1042/BJ2650891