Evidence that the Monoclonal Antibody an 51 is Directed Against Platelet Membrane Glycoprotein IB
作者： N. KIEFFER , C. RUAN , G. TOBELEM , A. McMICHAEL , J. CAEN
DOI: 10.1016/B978-0-08-029815-3.50089-1
关键词: Antibody 、 Platelet lysate 、 Antigen 、 Epitope 、 Molecular biology 、 Virology 、 Immunoprecipitation 、 Chemistry 、 Monoclonal antibody 、 Platelet 、 Glycoprotein Ib
摘要: Abstract An IgG monoclonal antibody (AN51) was produced against human platelets. The antigen, recognized by AN51 shown to be restricted platelets and megakaryocytes missing on from 6 different Bernard Soulier patients, deficient in platelet membrane Glycoprotein Ib (GP Ib). By immunoprecipitation, it that the antigen has a molecular weight of 150, 000 daltons. nature as GP confirmed crossed Immunoelectrophoresis using lysate, 125 I-AN51-F(ab′)2 rabbit anti-platelet IgG. Furthermore, epitope seems supported Is, soluble part Ib.
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